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Date: April 2005

Journal: Biological and Pharmaceutical Bulletin Volume 28, Issue 4, April 2005, Pages 661-666 , Doi: 10.1248/bpb.28.661

2005 | Inhibition of production of reactive oxygen species and gene expression profile by treatment of ethanol extract of Moutan Cortex Radicis in oxidative stressed PC12 cells…

Rho, S.a, Chung, H.-S.b, Kang, M.b, Lee, E.a, Cho, C.b, Kim, H.a, Park, S.a, Kim, H.-Y.a, Hong, M.a, Shin, M.a, Bae, H.ab

첨부파일

Abstract

Moutan Cortex Radicis (MCR) is one of the most widely used Oriental medicines. In this study, we assessed the reducing effect of ethanol extract of MCR on hydrogen peroxide-induced reactive oxygen production, the main cause of cell damage or death in PC12 cells. The viability of cells treated with 1 mg/ml of MCR was significantly restored from that of oxidative-stressed PC12 cells. Measurement of intracellular reactive oxygen species (ROS) generation was determined using the H2DCFDA assay. MCR at 1-0.01 mg/ml concentration inhibited ROS production in oxidative-stressed cells. To identify candidate genes responsible for the anti-oxidative effects of MCR on PC12 cells, an oligonucleotide microarray analysis was performed. The result of gene expression profiles showed that 10 genes were up-regulated and 7 were down-regulated in MCR plus hydrogen peroxide treated cells compared with hydrogen peroxide treated cells. Among them, heme oxygenase (HO) and cathechol-O-methyltransferase (COMT) are related to regulation of ROS generation and the others are known to regulate cell survival and progression. Subsequently, we performed real-time RT-PCR to quantify the ROS related gene. MCR treatment increased the expression of HO by 370% and COMT by 280% at the concentration of 1 mg/ml. These findings suggest that MCR inhibits the production of ROS and cytotoxicity by oxidative-stressed PC12 cells through over-expression of HO and COMT. © 2005 Pharmaceutical Society of Japan.


Author keywords

Cathechol-O-methyltransferase, heme oxygenase; Moutan cortex radicis; Oligonucleotide microarray; Reactive oxygen species


Indexed keywords

EMTREE drug terms: alcohol derivative; catechol methyltransferase; heme oxygenase; hydrogen peroxide; Paeonia suffruticosa extract; reactive oxygen metabolite

EMTREE medical terms: animal cell; antioxidant activity; article; cell damage; cell death; cell strain; cell survival; cell viability; concentration response; controlled study; cytotoxicity; DNA microarray; down regulation; drug isolation; gene control; gene expression; gene overexpression; nonhuman; nucleotide sequence; oxidative stress; Paeonia suffruticosa; rat; reverse transcription polymerase chain reaction; upregulation

MeSH: Animals; Down-Regulation; Gene Expression Profiling; Neurosecretory Systems; Oxidative Stress; Paeonia; PC12 Cells; Plant Extracts; Rats; Reactive Oxygen Species; Up-Regulation
Medline is the source for the MeSH terms of this document.


Molecular Sequence Numbers: GENBANK,AF020757(referenced), AF020758(referenced), AF028603(referenced), AI029917(referenced), AI179610(referenced), AJ222813(referenced), E12625(referenced), J02722(referenced), M74223(referenced), M93257(referenced), M99485(referenced), S47609(referenced), S68135(referenced), S73424(referenced), U08290(referenced), U14414(referenced), U77777(referenced), X06769(referenced), X07729(referenced), X16002(referenced)

Chemicals and CAS Registry Numbers: catechol methyltransferase, 9012-25-3; heme oxygenase, 9059-22-7; hydrogen peroxide, 7722-84-1;Plant Extracts; Reactive Oxygen Species


ISSN: 09186158 CODEN: BPBLESource Type: Journal Original language: English
DOI: 10.1248/bpb.28.661 PubMed ID: 15802806 Document Type: Article
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