Publications
기타논문
Date: July 2006
Journal: International Journal of Molecular Medicine Volume 18, Issue 1, July 2006, Pages 201-208 , Doi: 10.4111/kju.2006.47.2.201
2006 | Inhibition of cell cycle progression via p27Kip1 upregulation <b class="sch_word">an</b>d apoptosis induction by <b class="sch_word">an</b> eth<b class="sch_word">an</b>ol extract of Rhus verniciflua Stokes in AGS gastric c<b class="sch_word">an</b>cer cells…
- Kim, J.H.ac, Kim, H.-P.d, Jung, C.-H.a, Hong, M.H.ac, Hong, M.-C.b, Bae, H.-S.b, Lee, S.-D.e, Park, S.-Y.f, Park, J.-H.f, Ko, S.-G.ac
Abstract
Botanical preparations are widely used by patient with cancer in Korea, Japan and China. Rhus verniciflua Stokes (RVS) has traditionally been used as a medicinal ingredient for the therapy of stomach and uterine cancer. In this study, we showed that exposure to an ethanol extract of RVS (50 μg/ml) resulted in a synergistic inhibitory effect on cell growth in AGS cells. Growth inhibition was related with the inhibition of proliferation and induction of apoptosis. The extract induces G1-cell cycle arrest through the regulation of cyclins, the induction of p27Kip1, and decrease the CDK2 kinase activity. The upregulated p27Kip1 level is caused by protein stability increment by the reduction of Skp2, a key molecule related with p27Kip1 ubiquitination and degradation, and de novo protein synthesis. RVS extract induces apoptosis through the expression of Bax, poly(ADP-ribose) polymerase (PARP) and activation of caspase-3. RVS extract induces G1-cell cycle arrest via accumulation of p27Kip1 controlled by Skp2 reduction and apoptosis passing through an intrinsic pathway in human gastric cancer cells but not in normal cells, therefore we suggest that this extract could be a candidate medicine or compound for the development of novel class of anti-cancer drugs.
Author keywords
Apoptosis; Cell cycle; p27Kip1; Rhus verniciflua Stokes; Skp2
Indexed keywords
EMTREE drug terms: alcohol; CASP3 protein, human; caspase; caspase 3; cell cycle protein; cyclin dependent kinase inhibitor 1A; cyclin dependent kinase inhibitor 1B; messenger RNA; plant extract
EMTREE medical terms: apoptosis; article; cell cycle; cell division; cell line; cell survival; chemistry; dose response; drug effect; genetics; human; isolation and purification; metabolism; Northern blotting; pathology; physiology; poison ivy; stomach tumor; tumor cell line; upregulation
MeSH: Apoptosis; Blotting, Northern; Caspase 3; Caspases; Cell Cycle; Cell Cycle Proteins; Cell Division; Cell Line; Cell Line, Tumor; Cell Survival; Cyclin-Dependent Kinase Inhibitor p21; Cyclin-Dependent Kinase Inhibitor p27; Dose-Response Relationship, Drug; Ethanol; Humans; Plant Extracts; Rhus; RNA, Messenger; Stomach Neoplasms; Up-Regulation
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: alcohol, 64-17-5; caspase 3, 169592-56-7; caspase, 186322-81-6;CASP3 protein, human, EC 3.4.22.-; Caspase 3, EC 3.4.22.-; Caspases, EC 3.4.22.-; Cell Cycle Proteins; Cyclin-Dependent Kinase Inhibitor p21; Cyclin-Dependent Kinase Inhibitor p27, 147604-94-2; Ethanol, 64-17-5; Plant Extracts; RNA, Messenger