Date: 30 April 2007

Journal: Experimental and Molecular Medicine Volume 39, Issue 2, 30 April 2007, Pages 222-229 , Doi: 10.1038/emm.2007.25

2007 | Resveratrol stimulates glucose transport in C2C12 myotubes by activating AMP-activated protein kinase…

Chang, E.P.a, Kim, M.-J.b, Jong, H.L.a, Min, B.-I.a, Bae, H.c, Choe, W.b, Kim, S.-S.b, Ha, J.b


trans-Resveratrol (t-RVT), a naturally occurring polyphenol found in Polygonum cuspidatum, grape, and red wine, has been reported to have anti-inflammatory, cardioprotective, and cancer chemopreventive properties. However antidiabetic effect of t-RVT has not yet been reported. In this study, we show that t-RVT increases glucose uptake in C2C12 myotubes by activating AMP-activated protein kinase (AMPK), uncovering an antidiabetic potential of t-RVT for the first time. AMPK plays a central role in the regulation of glucose and lipid metabolism, and hence it is considered a novel therapeutic target for metabolic syndrome such as type 2 diabetes. t-RVT significantly induced glucose uptake in C2C12 cells, via AMPK activation, but not a phosphatidylinositol-3 kinase (PI-3 kinase) signal pathway. The induced glucose uptake was attenuated by pretreatment with a pharmacological inhibitor for AMPK, indicating that the effect of t-RVT primarily depends on AMPK activation. However, in the presence of insulin, t-RVT also potentiated the effect of insulin on glucose uptake via AMPK activation, which led to further activation of PI-3 kinase/Akt signal pathway.

Author keywords

1-phosphatidylinositol 3-kinase; 5′-AMP-activated protein kinase; Acetyl-CoA carboxylase; Glucose; Proto-oncogene proteins c-akt; Resveratrol

Indexed keywords

EMTREE drug terms: hydroxymethylglutaryl coenzyme A reductase kinase; insulin; phosphatidylinositol 3 kinase; resveratrol

EMTREE medical terms: animal cell; article; concentration response; controlled study; drug potentiation; enzyme activation; glucose transport; lipid metabolism; metabolic syndrome X; mouse; myotube; non insulin dependent diabetes mellitus; nonhuman; signal transduction

MeSH: 1-Phosphatidylinositol 3-Kinase; Animals; Biological Transport; Cell Line; Enzyme Activation; Glucose; Insulin; Mice; Models, Biological; Multienzyme Complexes; Muscle Fibers; Protein-Serine-Threonine Kinases; Proto-Oncogene Proteins c-akt; Stilbenes
Medline is the source for the MeSH terms of this document.

Species Index: Polygonum cuspidatum; Vitaceae

Chemicals and CAS Registry Numbers: hydroxymethylglutaryl coenzyme A reductase kinase, 172522-01-9, 72060-32-3; insulin, 9004-10-8; phosphatidylinositol 3 kinase, 115926-52-8; resveratrol, 501-36-0;1-Phosphatidylinositol 3-Kinase, EC; 5'-AMP-activated protein kinase, EC; Glucose, 50-99-7; Insulin, 11061-68-0; Multienzyme Complexes; Protein-Serine-Threonine Kinases, EC; Proto-Oncogene Proteins c-akt, EC; resveratrol, 501-36-0; Stilbenes

Manufacturers:Drug manufacturer: Sigma.

ISSN: 12263613 CODEN: EMMEFSource Type: Journal Original language: English
PubMed ID: 17464184 Document Type: Article
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