Moutan cortex (MC) is one of the most widely used Oriental herbal medicines for treating inflammatory diseases. In this study, the effect of MC on lipopolysaccharide (LPS) and recombinant interferon-gamma (rIFN-γ)-induced production of nitric oxide (NO) and tumor necrosis factor (TNF)-alpha were examined using mouse peritoneal macrophages. MC inhibited the LPS/rIFN-γ-induced expression of inducible nitric oxide synthase (iNOS) and TNF-alpha release. To clarify the mechanism involved, the effect of MC on the activation of nuclear factor (NF)-kappaB was examined. The LPS/rIFN-γ-induced activation of NF-kappaB was almost completely blocked by MC at 0.5 mg/ml. These findings demonstrate that the inhibition of the LPS/rIFN-γ-induced production of NO and TNF-alpha by MC is due to the inhibition of NF-kappaB activation. © 2007 Pharmaceutical Society of Japan.
Anti-inflammatory effect; Moutan cortex (MC); Nitric oxide; Peritoneal macrophage; Tumor necrosis factor-α
EMTREE drug terms: immunoglobulin enhancer binding protein; inducible nitric oxide synthase; nitric oxide; Paeonia suffruticosa extract; recombinant gamma interferon; tumor necrosis factor alpha
EMTREE medical terms: animal cell; article; controlled study; drug effect; drug inhibition; high performance liquid chromatography; mouse; nonhuman; Paeonia suffruticosa; peritoneum macrophage
MeSH: Animals; Anti-Inflammatory Agents, Non-Steroidal; Blotting, Western; Cell Nucleus; Cell Survival; Cells, Cultured; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Interferon-gamma, Recombinant; Lipopolysaccharides; Macrophage Activation; Macrophages, Peritoneal; Mice; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; Paeonia; Tumor Necrosis Factor-alpha
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: inducible nitric oxide synthase, 501433-35-8; nitric oxide, 10102-43-9;Anti-Inflammatory Agents, Non-Steroidal; Drugs, Chinese Herbal; Interferon-gamma, Recombinant; Lipopolysaccharides; moutan cortex; NF-kappa B; Nitric Oxide Synthase Type II, EC 18.104.22.168; Nitric Oxide, 10102-43-9; Tumor Necrosis Factor-alpha