본문

Inhibition of nitric oxide and tumor necrosis factor-alpha by moutan cortex in activated mouse peritoneal macrophages

  • AD 최고관리자
  • 조회 2346
  • 2014.12.16

Abstract

Moutan cortex (MC) is one of the most widely used Oriental herbal medicines for treating inflammatory diseases. In this study, the effect of MC on lipopolysaccharide (LPS) and recombinant interferon-gamma (rIFN-γ)-induced production of nitric oxide (NO) and tumor necrosis factor (TNF)-alpha were examined using mouse peritoneal macrophages. MC inhibited the LPS/rIFN-γ-induced expression of inducible nitric oxide synthase (iNOS) and TNF-alpha release. To clarify the mechanism involved, the effect of MC on the activation of nuclear factor (NF)-kappaB was examined. The LPS/rIFN-γ-induced activation of NF-kappaB was almost completely blocked by MC at 0.5 mg/ml. These findings demonstrate that the inhibition of the LPS/rIFN-γ-induced production of NO and TNF-alpha by MC is due to the inhibition of NF-kappaB activation. © 2007 Pharmaceutical Society of Japan.


Author keywords

Anti-inflammatory effect; Moutan cortex (MC); Nitric oxide; Peritoneal macrophage; Tumor necrosis factor-α


Indexed keywords

EMTREE drug terms: immunoglobulin enhancer binding protein; inducible nitric oxide synthase; nitric oxide; Paeonia suffruticosa extract; recombinant gamma interferon; tumor necrosis factor alpha

EMTREE medical terms: animal cell; article; controlled study; drug effect; drug inhibition; high performance liquid chromatography; mouse; nonhuman; Paeonia suffruticosa; peritoneum macrophage

MeSH: Animals; Anti-Inflammatory Agents, Non-Steroidal; Blotting, Western; Cell Nucleus; Cell Survival; Cells, Cultured; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Interferon-gamma, Recombinant; Lipopolysaccharides; Macrophage Activation; Macrophages, Peritoneal; Mice; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; Paeonia; Tumor Necrosis Factor-alpha
Medline is the source for the MeSH terms of this document.


Chemicals and CAS Registry Numbers: inducible nitric oxide synthase, 501433-35-8; nitric oxide, 10102-43-9;Anti-Inflammatory Agents, Non-Steroidal; Drugs, Chinese Herbal; Interferon-gamma, Recombinant; Lipopolysaccharides; moutan cortex; NF-kappa B; Nitric Oxide Synthase Type II, EC 1.14.13.39; Nitric Oxide, 10102-43-9; Tumor Necrosis Factor-alpha


ISSN: 09186158 CODEN: BPBLESource Type: Journal Original language: English
DOI: 10.1248/bpb.30.912 PubMed ID: 17473434 Document Type: Article
트위터 페이스북 미투데이 다음요즘 싸이공감 카카오톡 카카오스토리 네이트온 쪽지 구글 북마크 네이버 북마크

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