Our previous study demonstrated that successive electroacupuncture (EA) at ST36 acupoint reduces IgE production in BALB/c mice immunized with 2,4-dinitrophenylated keyhole limpet protein (DNP-KLH) by suppression of the Th2 cell lineage development. Here, we report that pretreatment of phentolamine (α-adrenoceptor antagonist, 10 mg/kg, i.p.) completely blocks the EA-induced suppression of antigen-specific and total IgE levels in serum and IL-4 production in anti-CD3 mAb-activated splenocytes in DNP-KLH immunized mice. The results suggest that α-adrenoceptor play an important role in mediating the suppressive effects of EA on IgE production and Th2 cell response in DNP-KLH immunized mice. © 2007 Elsevier Ireland Ltd. All rights reserved.
α-Adrenoceptor; DNP-KLH immunized mice; Electroacupuncture; IgE; IL-4
EMTREE drug terms: 2,4 dinitrophenyl keyhole limpet protein; alpha adrenergic receptor; immunoglobulin E; interleukin 4; monoclonal antibody CD3; phentolamine; unclassified drug; vaccine
EMTREE medical terms: animal cell; animal experiment; article; cell activation; controlled study; cytokine production; drug mechanism; electroacupuncture; female; immunization; immunoglobulin blood level; immunoglobulin production; immunomodulation; mouse; nonhuman; priority journal; spleen cell
MeSH: Adrenergic alpha-Antagonists; Animals; Electroacupuncture; Enzyme-Linked Immunosorbent Assay; Female; Hemocyanin; Immunoglobulin E; Interferon Type II; Interleukin-4; Mice; Mice, Inbred BALB C; Phentolamine; Receptors, Adrenergic, alpha; Th2 Cells
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: immunoglobulin E, 37341-29-0; phentolamine, 50-60-2, 73-05-2;Adrenergic alpha-Antagonists; Hemocyanin, 9013-72-3; Immunoglobulin E, 37341-29-0; Interferon Type II, 82115-62-6; Interleukin-4, 207137-56-2; Phentolamine, 50-60-2; Receptors, Adrenergic, alpha; keyhole-limpet hemocyanin