Abstract

Eosinophils have been implicated in a broad range of diseases, most notably allergic conditions (e.g. asthma, rhinitis and atopic dermatitis) and inflammatory diseases. These diseases are characterized by an accumulation of eosinophils in the tissue. Defining the mechanisms that control eosinophil recruitment is fundamental to understanding how these diseases progress and may identify a novel target for drug therapy. Eotaxin is a potent eosinophil-specific chemokine that is released in the respiratory epithelium after allergic stimulation. Aim of the study: In this study, we determined whether Moutan Cortex Radicis (MCR), a plant extract, effects eotaxin secretion from A549 epithelial cells and eosinophil chemotaxis, and then examined the mechanism involved. Materials and methods: Prior to assaying MCR's effects, A549 cells were stimulated with tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4) and IL-1β to induce expression of chemokines and adhesion molecules involved in eosinophil chemotaxis. In the presence of MCR, eotaxin, regulated on activation in normal T cells expressed and secreted (RANTES), IL-8, IL-16, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) transcripts were quantitated by real-time RT-PCR. Results: As a result, 0.01, 1, and 100 μg/ml of MCR treatments reduced eotaxin expression significantly and 0.01, 0.1, 1, 10, and 100 μg/ml of MCR reduced significantly eotaxin secretion. In addition, MCR treatment significantly inhibited eosinophil migration toward A549 medium. And 100 μg/ml of MCR suppressed the activated of nuclear factor (NF)-κB. Conclusions: These findings indicate that suppressed eotaxin secretion by MCR treatment is due to the inhibition of NF-κB activation. Therefore, MCR might be of therapeutic value in treating asthma. © 2007.

Author keywords

Chemotaxis; Eosinophil; Eotaxin; Moutan Cortex Radicis; NF-κB

Indexed keywords

EMTREE drug terms: cell adhesion molecule; chemokine; eotaxin; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; interleukin 16; interleukin 1beta; interleukin 4; interleukin 8; Paeonia radix; Paeonia suffruticosa extract; RANTES; tumor necrosis factor alpha; vascular cell adhesion molecule 1

EMTREE medical terms: allergy; article; asthma; cell migration; chemotaxis; concentration response; controlled study; cytokine production; drug mechanism; drug targeting; eosinophil; epithelium cell; human; human cell; leukocyte migration; Paeonia suffruticosa; real time polymerase chain reaction; respiratory epithelium; root cortex

MeSH: Blotting, Western; Cell Movement; Cell Nucleus; Cell Survival; Chemokine CCL5; Chemokines; Chemotaxis, Leukocyte; Chromatography, High Pressure Liquid; Cytokines; DNA, Complementary; Drugs, Chinese Herbal; Enzyme-Linked Immunosorbent Assay; Eosinophils; Epithelial Cells; Humans; Interleukin-1beta; Interleukin-4; Paeonia; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tumor Necrosis Factor-alpha
Medline is the source for the MeSH terms of this document.

Chemicals and CAS Registry Numbers: intercellular adhesion molecule 1, 126547-89-5; interleukin 8, 114308-91-7;Chemokine CCL5; Chemokines; Cytokines; DNA, Complementary; Drugs, Chinese Herbal; Interleukin-1beta; Interleukin-4, 207137-56-2; moutan cortex; RNA, Messenger; Tumor Necrosis Factor-alpha

Manufacturers:Drug manufacturer: Sun Ten, Taiwan.