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Date: 30 July 2009

Journal: Journal of Ethnopharmacology Volume 124, Issue 3, 30 July 2009, Pages 397-403 , Doi: 10.1016/j.jep.2009.05.036

2009 | Panax ginseng C.A. Meyer modulates the levels of MMP3 in S12 murine articular cartilage cell line…

Shin, J.-S.a, Park, N.b, Ra, J.c, Kim, Y.b, Shin, M.b, Hong, M.b, Kim, S.-H.d, Kwon, H.-J.c, Hong, S.-P.c, Kim, J.c , Bae, H.a

Abstract

Aim of the study: The destruction of cartilage in patients with osteoarthritis occurs due to an imbalance between matrix synthesis and degradation. Cartilage degradation is induced by the activation of matrix metalloproteinases (MMPs). Therefore, this study was conducted to evaluate the cartilage protective effect of Panax ginseng C.A. Meyer (PG). Materials and methods: S12 cells were treated with various concentrations of extract of PG and gensenosides Rd and Rb3 for 3 h, after which 10 ng/ml interleukin-1β (IL-1β) was added to the culture media. The levels of MMP3 in the conditioned media were then evaluated using an enzyme-linked immunosorbent assay (ELISA). In addition, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to evaluate the mRNA expression of Type II Collagen and Pro-collagenase. Furthermore, Western blot analysis was performed to identify the roles that PG played in the ERK and p38 signaling pathways. Results: The MMP3 secretion levels of S12 cells were significantly lowered in response to treatment with PG and gensenosides Rd and Rb3 at a concentration of 100 μg/ml when compared to cells that were treated with IL-1β. In addition, PG induced the mRNA expression of Type II Collagen dose dependently. Furthermore, phosphorylated p38 and ERK were detected in S12 articular cartilage cell line that was treated with IL-1β. PG decreased the phosphorylation of p38, but PG did not exert any effect on phospho-ERK. Conclusions: These findings indicate that PG and gensenosides Rd and Rb3 suppress MMP3 secretion and that gensenosides Rd and Rb3 are the major elements involved in the suppression of MMP3 by PG. Furthermore, the suppression of MMP3 by PG occurs via the inhibition of phospho-p38 activation. Therefore, PG may exert a protective effect against the cartilage degradation of OA. © 2009 Elsevier Ireland Ltd. All rights reserved.


Author keywords

Cartilage degradation; Matrix metalloproteinases; Panax ginseng C.A. Meyer; Phospho-p38


Indexed keywords

EMTREE drug terms: collagen type 2; ginseng extract; ginsenoside; ginsenoside rb 3; ginsenoside Rd; interleukin 1beta; messenger RNA; mitogen activated protein kinase; procollagenase; stromelysin; synaptophysin; unclassified drug

EMTREE medical terms: animal cell; article; articular cartilage; cartilage cell; cell protection; concentration response; controlled study; culture medium; enzyme inhibition; enzyme linked immunosorbent assay; enzyme regulation; enzyme release; gene expression; mouse; nonhuman; protein phosphorylation; reverse transcription polymerase chain reaction; signal transduction; Western blotting

MeSH: Blotting, Western; Cartilage, Articular; Cell Line; Cell Survival; Chromatography, High Pressure Liquid; Collagen Type II; Coloring Agents; Enzyme Inhibitors; Enzyme-Linked Immunosorbent Assay; Ginsenosides; Indicators and Reagents; Interleukin-1beta; Korea; Magnetic Resonance Spectroscopy; Matrix Metalloproteinase 3; Medicine, East Asian Traditional; p38 Mitogen-Activated Protein Kinases; Panax; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tetrazolium Salts; Thiazoles
Medline is the source for the MeSH terms of this document.

Species Index: Murinae; Panax ginseng


Chemicals and CAS Registry Numbers: ginsenoside, 74749-74-9; ginsenoside Rd, 52705-93-8; mitogen activated protein kinase, 142243-02-5; procollagenase, 9068-44-4; stromelysin, 79955-99-0;Collagen Type II; Coloring Agents; Enzyme Inhibitors; Ginsenosides; Indicators and Reagents; Interleukin-1beta; Matrix Metalloproteinase 3, 3.4.24.17; RNA, Messenger; Tetrazolium Salts; Thiazoles; ginsenoside Rb3, 68406-26-8; ginsenoside Rd, 52705-93-8; p38 Mitogen-Activated Protein Kinases, 2.7.11.24; thiazolyl blue, 298-93-1


ISSN: 03788741 CODEN: JOETDSource Type: Journal Original language: English
DOI: 10.1016/j.jep.2009.05.036 PubMed ID: 19505564 Document Type: Article
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