Abstract

Background/Aims: This study was conducted to evaluate the anti-inflammatory mechanisms of Erigeron canadensis (EC) on the tumor necrosis factor-α (TNF-α)-, interleukin (IL)-4- and IL-1β-induced stimulation of A549 cells. Methods: In the present study, the anti-inflammatory effects of EC on TNF-α-, IL-4- and IL-1β-induced A549 cells were determined by analyzing eotaxin secretion using ELISA. In addition, the effects of ECon gene expression profiles in stimulated A549 cells were evaluated by microarray analysis. Results: Oligonucleotide microarray analysis revealed that inflammatory-related genes such as NOS1, NOS2A, IL-1β, IL-8 and CSF2 and cell adhesion-related genes such as SELE, MMP3, VCAM1, ICAM1, ITGA7 and ITGB2 were downregulated in EC-treated A549 cells that had been pretreated with TNF-α, IL-4 and IL-1β. In addition, significant decreases in Eotaxin, ICAM, VCAM and IL-8 gene expression were observed in EC-treated A549 cells. Conclusions: EC has an anti-inflammatory effect in stimulated A549 cells. Microarray-based genomic survey is a high-throughput approach that is suitable for the evaluation of gene expression in cell lines that have been treated with EC. Copyright © 2009 S. Karger AG, Basel.

Author keywords

Asthma; Eotaxin; Erigeron canadensis; Gene expression profile; Interleukin-8; Intracellular adhesion molecule; Vascular cell adhesion molecule

Indexed keywords

EMTREE drug terms: alpha7 integrin; antiinflammatory agent; beta2 integrin; endothelial leukocyte adhesion molecule 1; eotaxin; Erigeron canadensis extract; granulocyte macrophage colony stimulating factor; inducible nitric oxide synthase; intercellular adhesion molecule 1; interleukin 1beta; interleukin 4; interleukin 8; neuronal nitric oxide synthase; plant extract; stromelysin; tumor necrosis factor alpha; unclassified drug; vascular cell adhesion molecule 1

EMTREE medical terms: antiinflammatory activity; article; cell adhesion; cell stimulation; controlled study; cytokine release; cytotoxicity; DNA microarray; down regulation; drug effect; drug mechanism; enzyme linked immunosorbent assay; epithelium cell; Erigeron; gene control; gene expression; genetic analysis; human; human cell; microarray analysis; priority journal

MeSH: Algorithms; Analysis of Variance; Anti-Inflammatory Agents, Non-Steroidal; Cell Line, Tumor; Chemokine CCL11; Cytokines; Dietary Supplements; Epithelial Cells; Erigeron; Gene Expression Regulation; Humans; Intercellular Adhesion Molecule-1; Interleukin-1beta; Interleukin-4; Interleukin-8; MAP Kinase Signaling System; Oligonucleotide Array Sequence Analysis; Plant Extracts; Pulmonary Alveoli; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tumor Necrosis Factor-alpha; Vascular Cell Adhesion Molecule-1
Medline is the source for the MeSH terms of this document.

Species Index: Conyza canadensis canadensis

Chemicals and CAS Registry Numbers: beta2 integrin, 172592-43-7; endothelial leukocyte adhesion molecule 1, 128875-25-2; inducible nitric oxide synthase, 501433-35-8; intercellular adhesion molecule 1, 126547-89-5; interleukin 8, 114308-91-7; neuronal nitric oxide synthase, 506430-87-1; stromelysin, 79955-99-0;Anti-Inflammatory Agents, Non-Steroidal; Chemokine CCL11; Cytokines; Intercellular Adhesion Molecule-1, 126547-89-5; Interleukin-1beta; Interleukin-4, 207137-56-2; Interleukin-8; Plant Extracts; RNA, Messenger; Tumor Necrosis Factor-alpha; Vascular Cell Adhesion Molecule-1