Abstract

The goal of this study was to quantitatively determine the recovery effects of herbal medicines (HM) on the cisplatin-induced nephrotoxicity. In the present study, the recovery effects of 239 HM on HEK 293 cells that had been damaged by cisplatin were evaluated by a mitochondrial activity MTS assay. After the first round of screening, candidate HM were selected based on a recovery rate of greater than 20%. The efficacy of the selected herbs was then determined by dose response kinetic analysis. Of the extracts evaluated, 7 HM (Paeonia suffruticosa (PS), Curcuma longa (CL), Centipeda minima (CM), Loranthus parasiticus (LP), Pulsatilla dahurica (PD), Sinapis alba (SA), and Scutellaria barbata (SB)) had a strong recovery effect on cisplatin-induced damage in HEK 293 cells. An LDH assay showed that LP, CM, SB, CL, SA, and PS had the best recovery effect, whereas a comet assay indicated that PS, SB, SA, PD, and CL had the best recovery effect. Taken together, these results suggest that SB, CL, PS, and SA are the best candidate HM for the recovery of cisplatin-induced nephrotoxicity. Therefore, additional studies should be conducted to determine if these HM possess novel therapeutic agents that can be used for the prevention or treatment of renal disorders. © 2009 Elsevier B.V. All rights reserved.

Author keywords

Cisplatin; Curcuma longa; Herbal medicines; Nephrotoxicity; Paeonia suffruticosa; Scutellaria barbata

Indexed keywords

EMTREE drug terms: Agrimonia pilosa extract; Anemarrhena asphodeloides extract; Angelica sinensis extract; Atractylodes extract; Bupleurum root extract; Centipeda minima extract; cisplatin; Clematis chinensis extract; Curcuma longa extract; Cuscuta chinensis extract; Ephedra sinica extract; Evodia fruit extract; Gardenia jasminoides extract; ginseng extract; herbaceous agent; Houttuynia cordata extract; lactate dehydrogenase; Lonicera japonica extract; Loranthus parasiticus extract; Ocimum basilicum extract; Paeonia suffruticosa extract; Poria cocos extract; Pulsatilla dahurica extract; Rehmannia glutinosa extract; Salvia miltiorrhiza extract; Schisandra chinensis extract; Scutellaria barbata extract; Sinapis alba extract; unclassified drug; unindexed drug; Vitex rotundifolia extract

EMTREE medical terms: animal experiment; article; cell strain HEK293; Centipeda minima; controlled study; drug efficacy; drug mechanism; drug screening; herbal medicine; human; human cell; IC 50; Loranthus parasiticus; male; medicinal plant; mouse; nephrotoxicity; nonhuman; priority journal; Pulsatilla dahurica; renal protection; Scutellaria barbata; sinapis alba

Species Index: Centipeda minima; Curcuma longa; Loranthus parasiticus; Paeonia suffruticosa; Pulsatilla; Scutellaria barbata; Sinapis alba

Chemicals and CAS Registry Numbers: cisplatin, 15663-27-1, 26035-31-4, 96081-74-2; lactate dehydrogenase, 9001-60-9

Manufacturers:Drug manufacturer: Sigma, United States;Sun Ten, Taiwan.