Aim of the study: The root of Scutellaria baicalensis Georgi (Labiatae), also known as Scutellariae Radix, possesses anticancer, antiviral, and anti-inflammatory properties. And it is one of the most widespread herbal remedies used in Oriental medicine. In the present study, we investigated the effects of Scutellariae Radix water extract (SR) on proinflammatory mediators secreted from lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Material and methods: Cell viability was assessed by MTT assay and nitric oxide (NO) concentration in the cultured medium was determined by the Griess reaction. Various Cytokines released from LPS-induced Raw 264.7 cells were measured in the cell culture supernatants using a multiplex bead array assay based on xMAP technology. Results: We found that SR significantly inhibited the production of NO, interleukin (IL)-3, IL-6, IL-10, IL-12p40, IL-17, interferon-inducible protein (IP)-10, keratinocyte-derived chemokine (KC), and vascular endothelial growth factor (VEGF) in LPS-induced RAW 264.7 cells at the concentrations of 25, 50, 100, 200 μg/ml (p < 0.05). Conclusions: These results suggest that SR has anti-inflammatory activity related with its inhibition of NO, cytokine, chemokine, and growth factor production in macrophages. © 2009 Elsevier Ireland Ltd. All rights reserved.
Anti-inflammatory; Cytokine; Labiatae; Macrophage; Nitric oxide; Scutellaria baicalensis
EMTREE drug terms: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; antiinflammatory agent; cytokine; gamma interferon inducible protein 10; interleukin 10; interleukin 12p40; interleukin 17; interleukin 3; interleukin 6; keratinocyte derived chemokine; lipopolysaccharide; nitric oxide; Scutellaria baicalensis extract; vasculotropin; water
EMTREE medical terms: animal cell; antiinflammatory activity; article; cell culture; cell line; cell viability; concentration response; controlled study; culture medium; cytokine production; cytokine release; drug isolation; drug mechanism; macrophage; mouse; nonhuman; plant root; protein synthesis inhibition; Scutellaria baicalensis; supernatant
MeSH: Animals; Anti-Inflammatory Agents; Cells, Cultured; Cytokines; Inflammation Mediators; Lipopolysaccharides; Macrophages; Mice; Nitric Oxide; Plant Extracts; Plant Roots; Scutellaria baicalensis; Vascular Endothelial Growth Factor A
Medline is the source for the MeSH terms of this document.
Species Index: Lamiaceae; Scutellaria baicalensis; Scutellaria radix
Chemicals and CAS Registry Numbers: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, 298-93-1; gamma interferon inducible protein 10, 97741-20-3; nitric oxide, 10102-43-9; vasculotropin, 127464-60-2; water, 7732-18-5;Anti-Inflammatory Agents; Cytokines; Inflammation Mediators; Lipopolysaccharides; Nitric Oxide, 10102-43-9; Plant Extracts; Scutellaria baicalensis extract; Vascular Endothelial Growth Factor A