Objectives: Agrimoniae Herba has been used as an anti-inflammatory agent in traditional medicine. Nitric oxide and proinflammatory cytokines produced by activated microglia may be a possible etiological factor of neurodegenerative disorders. We evaluated whether Agrimoniae Herba could have an anti-inflammatory effect on lipopolysaccharide-induced BV2 microglial cells. Methods: The effects of Agrimoniae Herba on lipopolysaccharide-induced nitric oxide and proinflammatory cytokine production in BV2 microglial cells were evaluated by nitric oxide assay, enzyme-linked immunosorbent assay and western blotting. Results: Agrimoniae Herba had no cytotoxicity and suppressed lipopolysaccharide-induced nitric oxide production in BV2 microglial cells. Agrimoniae Herba also suppressed lipopolysaccharide-induced production of proinflammatory cytokines such as tumor necrosis factor, interleukin 1 beta and interleukin 6 in a dose-dependent manner. Agrimoniae Herba inhibited the expression of inducible nitric oxide synthase. Discussion: Taken together, these findings indicate that Agrimoniae Herba may be used as a form of pharmaceutical acupuncture therapy in the treatment of brain inflammation. [Neurol Res 2010; 32: 53-57. © 2010 W. S. Maney & Son Ltd.
Agrimoniae Herba; BV2 microglial cells; Nitric oxide; Pharmaceutical acupuncture; Proinflammatory cytokines
EMTREE drug terms: Agrimoniae herba extract; inducible nitric oxide synthase; interleukin 1beta; interleukin 6; lipopolysaccharide; nitric oxide; plant extract; unclassified drug
EMTREE medical terms: agrimoniae herba; animal cell; antiinflammatory activity; article; controlled study; cytokine production; cytotoxicity; drug inhibition; enzyme inhibition; enzyme linked immunosorbent assay; herbal medicine; inflammation; medicinal plant; microglia; mouse; nonhuman; protein expression; Western blotting
MeSH: Agrimonia; Animals; Anti-Inflammatory Agents; Cell Death; Cell Line; Chromatography, High Pressure Liquid; Cytokines; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Ellagic Acid; Enzyme-Linked Immunosorbent Assay; Immunoblotting; Interleukin-1beta; Interleukin-6; Lipopolysaccharides; Mice; Microglia; Nitric Oxide; Nitric Oxide Synthase Type II; Tumor Necrosis Factor-alpha
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: inducible nitric oxide synthase, 501433-35-8; nitric oxide, 10102-43-9;Anti-Inflammatory Agents; Cytokines; Drugs, Chinese Herbal; Ellagic Acid, 476-66-4; Interleukin-1beta; Interleukin-6; Lipopolysaccharides; Nitric Oxide, 10102-43-9; Nitric Oxide Synthase Type II, 188.8.131.52; Nos2 protein, mouse, 184.108.40.206; Tumor Necrosis Factor-alpha
Manufacturers:Drug manufacturer: Sun Ten, Taiwan.