Abstract
Previously, we demonstrated that electoracupuncture (EA) suppressed allergic reactions in DNP-KLH immunized mice. In this study, the mechanisms by which EA induces immunomodulation in the immunized mice were evaluated by genome-wide microarray analysis. The anti-allergic effects of EA in DNP-KLH immunized mice were confirmed by analyzing antigen specific IgE using ELISA. Microarray analysis, followed by real time RT-PCR validation, revealed that Th1 and Th17 cytokine-, opioid peptide-, and anti-apoptosis-related genes were up-regulated upon treatment with EA. In addition, significant decreases in Th2 cytokine-, MAPK signaling pathway-, and apoptosisrelated genes were observed following EA treatment. © Springer Science+Business Media, LLC 2009.
Author keywords
Electroacupuncture; Gene expression profile; IgE; Microarray
Indexed keywords
EMTREE drug terms: 2,4 dinitrophenol; immunoglobulin E; keyhole limpet hemocyanin; mitogen activated protein kinase
EMTREE medical terms: animal experiment; animal tissue; apoptosis; article; controlled study; electroacupuncture; enzyme linked immunosorbent assay; female; gene cluster; gene expression profiling; genetic association; immunization; immunomodulation; microarray analysis; mouse; nonhuman; priority journal; real time polymerase chain reaction; reverse transcription polymerase chain reaction; signal transduction; Th2 cell; upregulation
MeSH: 2,4-Dinitrophenol; Adjuvants, Immunologic; Animals; Cytokines; Electroacupuncture; Female; Gene Expression Profiling; Genome; Hemocyanin; Humans; Hypersensitivity, Immediate; Immunoglobulin E; Mice; Mice, Inbred BALB C; Multigene Family; Oligonucleotide Array Sequence Analysis; Random Allocation; Reproducibility of Results; Uncoupling Agents
Medline is the source for the MeSH terms of this document.
Species Index: Mus
Chemicals and CAS Registry Numbers: 2,4 dinitrophenol, 25550-58-7, 51-28-5; immunoglobulin E, 37341-29-0; mitogen activated protein kinase, 142243-02-5;2,4-Dinitrophenol, 51-28-5; Adjuvants, Immunologic; Cytokines; Hemocyanin, 9013-72-3; Immunoglobulin E, 37341-29-0; Uncoupling Agents; keyhole-limpet hemocyanin
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