Date: 2010

Journal: Bioscience, Biotechnology and Biochemistry Volume 74, Issue 6, 2010, Pages 1152-1156 , Doi: 10.1271/bbb.90676

2010 | Inhibitory effects of Paeonia suffruticosa on allergic reactions by inhibiting the NF-kappaB/IkappaB-alpha signaling pathway and phosphorylation of ERK in an animal model and human mast cells…

Hong, M.H.a, Kim, J.H.b, Na, S.H.a, Bae, H.c, Shin, Y.C.a, Kim, S.H.b, Ko, S.G.abd


The root cortex of Paeonia sujfruticosa Andrews (PSA), also known as Moutan Cortex, is known to have anti-allergic and anti-inflammatory properties. This study investigates the effect and mechanism of PSA by in vivo and in vitro methods. Treatings the root cortex from PSA with up to 0.4mg/ml of an ethanol extract showed no cytotoxicity in human mast cells. The ethanol extract of PSA (200mn/kg) significantly inhibited the passive cutaneous anaphylaxis reaction in vivo, and suppressed the release of histamine from rat peritoneal mast cells induced by compound 48/80. It was also found that PSA decreased the expressions of TNF-alpha and IL-6 in PMA- and A23187-stimulated HMC-1 cells. The results show the inactivation of IkappaB-alpha and NF-kappaB, as well as suppression of the phosphorylation of extracellular signal-regulated kinase (ERK). Our findings therefore suggest that PSA could be promising for anti-allergic inflammation by inhibiting the NF- kappaB/IkappaB-alpha signaling pathway and the phosphorylation of ERK.

Author keywords

Allergic reaction; ERK; Mast cells; NF-kappaB; Paeonia suffruticosa Andrews

Indexed keywords

Allergic inflammation; Allergic reactions; Animal model; Anti-inflammatories; Ethanol extract; Extracellular signal-regulated kinase; Human mast cells; In-vitro; In-vivo; Inhibitory effect; Mast cells; NF-kappaB; Signaling pathways

Engineering controlled terms: Ethanol; Signaling

Engineering main heading: Phosphorylation

EMTREE drug terms: antiinflammatory agent; I kappa B; immunoglobulin E; immunoglobulin enhancer binding protein; interleukin 6; mitogen activated protein kinase; NF kappaB inhibitor alpha; NF-kappaB inhibitor alpha; plant extract; tumor necrosis factor alpha

EMTREE medical terms: animal; animal model; article; biosynthesis; chemistry; drug effect; histamine release; human; hypersensitivity; immunology; male; mast cell; metabolism; Paeonia; phosphorylation; rat; signal transduction; Sprague Dawley rat

MeSH: Animals; Anti-Inflammatory Agents; Extracellular Signal-Regulated MAP Kinases; Histamine Release; Humans; Hypersensitivity; I-kappa B Proteins; Immunoglobulin E; Interleukin-6; Male; Mast Cells; Models, Animal; NF-kappa B; Paeonia; Phosphorylation; Plant Extracts; Rats; Rats, Sprague-Dawley; Signal Transduction; Tumor Necrosis Factor-alpha
Medline is the source for the MeSH terms of this document.

Species Index: Animalia; Paeonia; Paeonia suffruticosa; Rattus

Chemicals and CAS Registry Numbers: immunoglobulin E, 37341-29-0; mitogen activated protein kinase, 142243-02-5;Anti-Inflammatory Agents; Extracellular Signal-Regulated MAP Kinases,; I-kappa B Proteins; Immunoglobulin E, 37341-29-0; Interleukin-6; NF-kappa B; NF-kappaB inhibitor alpha, 139874-52-5; Plant Extracts; Tumor Necrosis Factor-alpha

ISSN: 09168451 CODEN: BBBIESource Type: Journal Original language: English
DOI: 10.1271/bbb.90676 PubMed ID: 20530912 Document Type: Article
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