Abstract

The root cortex of Paeonia sujfruticosa Andrews (PSA), also known as Moutan Cortex, is known to have anti-allergic and anti-inflammatory properties. This study investigates the effect and mechanism of PSA by in vivo and in vitro methods. Treatings the root cortex from PSA with up to 0.4mg/ml of an ethanol extract showed no cytotoxicity in human mast cells. The ethanol extract of PSA (200mn/kg) significantly inhibited the passive cutaneous anaphylaxis reaction in vivo, and suppressed the release of histamine from rat peritoneal mast cells induced by compound 48/80. It was also found that PSA decreased the expressions of TNF-alpha and IL-6 in PMA- and A23187-stimulated HMC-1 cells. The results show the inactivation of IkappaB-alpha and NF-kappaB, as well as suppression of the phosphorylation of extracellular signal-regulated kinase (ERK). Our findings therefore suggest that PSA could be promising for anti-allergic inflammation by inhibiting the NF- kappaB/IkappaB-alpha signaling pathway and the phosphorylation of ERK.

Author keywords

Allergic reaction; ERK; Mast cells; NF-kappaB; Paeonia suffruticosa Andrews

Indexed keywords

Allergic inflammation; Allergic reactions; Animal model; Anti-inflammatories; Ethanol extract; Extracellular signal-regulated kinase; Human mast cells; In-vitro; In-vivo; Inhibitory effect; Mast cells; NF-kappaB; Signaling pathways

Engineering controlled terms: Ethanol; Signaling

Engineering main heading: Phosphorylation

EMTREE drug terms: antiinflammatory agent; I kappa B; immunoglobulin E; immunoglobulin enhancer binding protein; interleukin 6; mitogen activated protein kinase; NF kappaB inhibitor alpha; NF-kappaB inhibitor alpha; plant extract; tumor necrosis factor alpha

EMTREE medical terms: animal; animal model; article; biosynthesis; chemistry; drug effect; histamine release; human; hypersensitivity; immunology; male; mast cell; metabolism; Paeonia; phosphorylation; rat; signal transduction; Sprague Dawley rat

MeSH: Animals; Anti-Inflammatory Agents; Extracellular Signal-Regulated MAP Kinases; Histamine Release; Humans; Hypersensitivity; I-kappa B Proteins; Immunoglobulin E; Interleukin-6; Male; Mast Cells; Models, Animal; NF-kappa B; Paeonia; Phosphorylation; Plant Extracts; Rats; Rats, Sprague-Dawley; Signal Transduction; Tumor Necrosis Factor-alpha
Medline is the source for the MeSH terms of this document.

Species Index: Animalia; Paeonia; Paeonia suffruticosa; Rattus

Chemicals and CAS Registry Numbers: immunoglobulin E, 37341-29-0; mitogen activated protein kinase, 142243-02-5;Anti-Inflammatory Agents; Extracellular Signal-Regulated MAP Kinases, 2.7.11.24; I-kappa B Proteins; Immunoglobulin E, 37341-29-0; Interleukin-6; NF-kappa B; NF-kappaB inhibitor alpha, 139874-52-5; Plant Extracts; Tumor Necrosis Factor-alpha