Abstract
Four known alkyl 4-hydroxybenzoates, i.e., methyl 4-hydroxybenzoate (1), ethyl 4-hydroxybenzoate (2), propyl 4-hydroxybenzoate (3), and butyl 4-hydroxybenzoate (4), were isolated from the seeds of Nelumbo nucifera GAERTNER (Nymphaeaceae) for the first time. The structures of the isolates were identified by 1D- and 2D-NMR spectroscopy and comparison with published values. The compounds were evaluated for their effects on the 5-HT-stimulated inward current (I5-HT) mediated by the human 5-HT3A receptors expressed in Xenopus oocytes. Compounds 1 and 2 enhanced the I5-HT, but 4 reduced it. These results indicate that 4 is an inhibitor of the 5-HT 3A receptors expressed in Xenopus oocytes. © 2010 Verlag Helvetica Chimica Acta AG.
Indexed keywords
EMTREE drug terms: butyl paraben; diltiazem; ethyl paraben; hydroxybenzoic acid derivative; methyl paraben; Nelumbo nucifera extract; propyl paraben; serotonin 3A receptor; hydroxybenzoic acid derivative; serotonin 3 agonist; serotonin 3 antagonist; serotonin 3 receptor
EMTREE medical terms: animal cell; article; channel gating; controlled study; drug receptor binding; human; IC 50; nonhuman; oocyte; plant seed; structure activity relation; Xenopus; animal; cell culture; cell membrane potential; chemical structure; chemistry; dose response; drug effect; genetic transfection; genetics; isolation and purification; metabolism; Nelumbo; Xenopus laevis
Species Index: Nelumbo nucifera; Nymphaeaceae
MeSH: Animals; Cells, Cultured; Dose-Response Relationship, Drug; Humans; Hydroxybenzoic Acids; Membrane Potentials; Molecular Structure; Nelumbo; Oocytes; Receptors, Serotonin, 5-HT3; Seeds; Serotonin 5-HT3 Receptor Agonists; Serotonin 5-HT3 Receptor Antagonists; Structure-Activity Relationship; Transfection; Xenopus laevis
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: butyl paraben, 94-26-8; diltiazem, 33286-22-5, 42399-41-7; ethyl paraben, 120-47-8; methyl paraben, 99-76-3; propyl paraben, 94-13-3;Hydroxybenzoic Acids; Receptors, Serotonin, 5-HT3; Serotonin 5-HT3 Receptor Agonists; Serotonin 5-HT3 Receptor Antagonists
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