To evaluate the direct effects of Cyperi Rhizoma (CR), a plant water extract, on Th1/Th2 lineage development in vitro, this study was conducted. Sorted CD4+ T cells obtained from the splenocytes of BALB/c mice were activated with anti-CD3/anti-CD28 and then cultured in medium that contained CR medium under Th1 inducing or Th2 inducing conditions. Subsequently, IFN-γ or IL-4 secreting cells were quantitated using flow cytometry analysis. In addition, IFN-γ and IL-4 protein secretions were detected by ELISA analysis, after which, IFN-γ and T-bet transcripts, key players in the Th1 immune function, and also, IL-4 and GATA-3, which are primary components in the Th2 immune mechanism, were quantitated by real-time RT-PCR. CR had no mitogenic effects on un-stimulated CD4+ T cells, however, it increased the CD4+ T cell population. Th1/Th2 polarization experiments revealed that CR enhanced IFN-γ secretion in Th1 cells, but reduced the IL-4 in Th2 cells, and this occurred in a dose-dependent manner and showed significances. In addition, under Th1/Th2 skewed conditions, the transcription levels of IFN-γ and T-bet were considerably increased, while the expressions of IL-4 and GATA-3 were relatively decreased with CR treatment. These findings suggest that CR enhances Th1 lineage development by increasing Th1 specific cytokine expression and secretion and reduces Th2 lineage development by repressing Th2 specific cytokine productions. Therefore, CR extract may be useful for preventing the onset of allergies or improving allergic symptoms. © 2010 Elsevier Ltd.
Cyperi Rhizoma; IFN-γ; IL-4; Th1; Th2
EMTREE drug terms: CD4 antigen; Cyperus rotundus extract; eugenol; gamma interferon; glyceraldehyde 3 phosphate dehydrogenase; interleukin 4; monoclonal antibody CD28; monoclonal antibody CD3; transcription factor GATA 3; transcription factor T bet
EMTREE medical terms: allergy; animal cell; article; CD4+ T lymphocyte; cell activation; cell culture; cell lineage; cell maturation; cell proliferation; cell survival; cell viability; controlled study; Cyperus; Cyperus rotundus; cytokine production; cytokine release; enzyme linked immunosorbent assay; female; flow cytometry; immune response; in vitro study; mouse; nonhuman; plant root; priority journal; protein expression; reverse transcription polymerase chain reaction; spleen cell; T lymphocyte subpopulation; Th1 cell; Th2 cell
MeSH: Animals; Cell Lineage; Cell Proliferation; Cell Survival; Chromatography, High Pressure Liquid; Cyperus; Eugenol; GATA3 Transcription Factor; Gene Expression Regulation; Interferon-gamma; Interleukin-4; Mice; Plant Extracts; Rhizome; RNA, Messenger; T-Box Domain Proteins; Th1 Cells; Th2 Cells
Medline is the source for the MeSH terms of this document.
Species Index: Mus
Chemicals and CAS Registry Numbers: eugenol, 97-53-0; gamma interferon, 82115-62-6; glyceraldehyde 3 phosphate dehydrogenase, 9001-50-7; transcription factor GATA 3, 137878-55-8;Eugenol, 97-53-0; GATA3 Transcription Factor; Gata3 protein, mouse; Interferon-gamma, 82115-62-6; Interleukin-4, 207137-56-2; Plant Extracts; RNA, Messenger; T-Box Domain Proteins; T-box transcription factor TBX21