Background: Platycodon grandiflorum is a traditional Asian medicine that is used to treat pulmonary and respiratory allergic disorders. Objective: To investigate the effects of P grandiflorum in vivo in an animal model of atopic dermatitis (AD), with particular emphasis on its effects on TH1 and TH2 immune responses. Methods: We established a model of AD-like skin lesions in NC/Nga mice. After oral administration of P grandiflorum, we measured cytokine and immunoglobulin profiles along with histologic examination of skin. Results: P grandiflorum was nontoxic in a 2,4-dinitrofluorobenzene- induced model of AD-like skin lesions in NC/Nga mice. AD symptoms in skin lesions improved after oral administration of P grandiflorum. IgE secretion was significantly downregulated in P grandiflorum-treated animals, accompanied by decreased levels of interleukin (IL) 4 and IgG1 and increased serum levels of IL-12p40 and IgG2a. In isolated splenocytes, the production of the T H1 cytokines IL-12p40 and interferon-γ was upregulated by P grandiflorum, whereas the levels of the TH2 cytokines IL-4 and IL-5 were downregulated in a mouse model of AD-like skin lesions. Conclusions: These results suggest that P grandiflorum inhibits the development of AD-like skin lesions in NC/Nga mice by suppressing the TH2 cell response and increasing the TH1 cell responses. Our results indicate that P grandiflorum is safe and effective as a natural herbal medicine for the treatment of AD-like skin lesions. © 2011 American College of Allergy, Asthma & Immunology.
EMTREE drug terms: dexamethasone; gamma interferon; immunoglobulin E; immunoglobulin G1; immunoglobulin G2a; interleukin 12p40; interleukin 4; interleukin 5; Platycodon grandiflorum extract
EMTREE medical terms: animal cell; animal experiment; animal model; animal tissue; article; atopic dermatitis; cell isolation; cellular immunity; controlled study; cytokine production; down regulation; drug effect; drug efficacy; drug safety; herbal medicine; histopathology; immunoglobulin production; in vivo study; male; mouse; nonhuman; priority journal; spleen cell; Th1 cell; Th2 cell; upregulation
MeSH: Animals; Dermatitis, Atopic; Dinitrofluorobenzene; Disease Models, Animal; Immunoglobulin E; Immunoglobulin G; Male; Mice; Phytotherapy; Plant Extracts; Platycodon; Skin; Th1 Cells; Th2 Cells
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: dexamethasone, 50-02-2; gamma interferon, 82115-62-6; immunoglobulin E, 37341-29-0;Dinitrofluorobenzene, 70-34-8; Immunoglobulin E, 37341-29-0; Immunoglobulin G; Plant Extracts