Date: 14 July 2011

Journal: Journal of Ethnopharmacology Volume 136, Issue 3, 14 July 2011, Pages 399-405 , Doi: 10.1016/j.jep.2011.01.014

2011 | Inhibitory effects of casticin on migration of eosinophil and expression of chemokines and adhesion molecules in A549 lung epithelial cells via NF-κB inactivation…

Koh, D.-J.ab, Ahn, H.-S.b, Chung, H.-S.a, Lee, H.a, Kim, Y.c, Lee, J.-Y.b, Kim, D.-G.b, Hong, M.b, Shin, M.b , Bae, H.b


Ethnopharmacological relevance: The fruits of Vitex rotundifolia L. have long been used for the treatment of inflammation of the respiratory tract in East Asia. Aim: To determine if casticin, one of the constituents of Vitex rotundifolia L., has anti-allergic and anti-inflammatory effects in asthma. Materials and methods: The in vitro anti-inflammatory activity of casticin was studied in A549 human type II-like epithelial lung cells using an eotaxin inhibition assay. Additionally, its effects on eotaxin, regulated on activation normal T cell expressed and secreted (RANTES), vascular cell adhesion molecule (VCAM)-1, and inter-cellular adhesion molecule (ICAM)-1 expression were investigated by real time-polymerase chain reaction (real time-PCR). The inhibition of nuclear factor κB (NF-κB) activity in the presence of casticin was determined by analyzing confocal microscopy images of fluorescence immunocytochemical analysis while the suppression of inhibitory κB (IκB)-α phosphorylation was studied using Western blot analysis. Finally, the inhibitory effect of casticin on eosinophil migration toward prestimulated A549 cell media was measured using the human eosinophilic leukemia cell line. Results and discussion: Casticin significantly suppressed eotaxin production in cytokine activated A549 lung epithelial cells. Casticin also suppressed the mRNA expression levels of eotaxin, RANTES, VCAM-1, and ICAM-1, which subsequently contributed to the inhibition of eosinophil migration. Furthermore, casticin inhibited IκB-α phosphorylation and nuclear translocation of p65 in A549 cells. Conclusion: Casiticin inhibited the eosinophil migration and activity of chemokines and adhesion molecules involved in the inflammatory process of asthma by suppressing the NF-κB pathway. These results suggest that casticin has the potential for use in the treatment of allergic asthma. © 2011 Elsevier Ireland Ltd.

Author keywords

Asthma; Casticin; Eosinophil; Inflammation; NF-κB

Indexed keywords

EMTREE drug terms: casticin; cell adhesion molecule; chemokine; eotaxin; I kappa B alpha; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; RANTES; synaptotagmin I; unclassified drug; vascular cell adhesion molecule 1; Vitex rotundifolia extract

EMTREE medical terms: allergic asthma; antiinflammatory activity; article; asthma; cell migration; confocal microscopy; eosinophil; fruit; human; human cell; leukemia cell line; lung alveolus epithelium; protein localization; protein phosphorylation; real time polymerase chain reaction; respiratory tract inflammation; Vitex rotundifolia; Western blotting

MeSH: Anti-Inflammatory Agents; Asthma; Cell Adhesion Molecules; Cell Line; Chemokines, CC; Chemotaxis, Leukocyte; Cytokines; Eosinophils; Epithelial Cells; Flavonoids; Fruit; Humans; I-kappa B Proteins; Lung; NF-kappa B; Phytotherapy; Plant Extracts; RNA, Messenger; Transcription Factor RelA; Vitex
Medline is the source for the MeSH terms of this document.

Species Index: Vitex rotundifolia

Chemicals and CAS Registry Numbers: I kappa B alpha, 151217-48-0; casticin, 479-91-4; intercellular adhesion molecule 1, 126547-89-5;Anti-Inflammatory Agents; Cell Adhesion Molecules; Chemokines, CC; Cytokines; Flavonoids; I-kappa B Proteins; NF-kappa B; NF-kappaB inhibitor alpha, 139874-52-5; Plant Extracts; RNA, Messenger; Transcription Factor RelA; casticin, 479-91-4

ISSN: 03788741 CODEN: JOETDSource Type: Journal Original language: English
DOI: 10.1016/j.jep.2011.01.014 PubMed ID: 21251967 Document Type: Article
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