Date: August 2011

Journal: Molecules and Cells Volume 32, Issue 2, August 2011, Pages 123-132 , Doi: 10.1007/s10059-011-2254-1

2011 | The genome-wide expression profile of 1,2,3,4,6-penta-O-galloyl-ß-D- glucose-treated MDA-MB-231 breast cancer cells: Molecular target on cancer metabolism…

Yu, W.S.a, Jeong, S.-J.a, Kim, J.-H.a, Lee, H.-J.a, Song, H.S.a, Kim, M.-S.b, Ko, E.a, Lee, H.-J.a, Khil, J.-H.c, Jang, H.-J.a, Kim, Y.C.a, Bae, H.a, Chen, C.Y.d, Kim, S.-H.a


1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphenolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the significant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes. ©2011 KSMCB.

Author keywords

Cancer metabolism; MDA-MB-231; Microarray; PGG; Real-time PCR

Indexed keywords

EMTREE drug terms: 1,2,3,4,6 penta o galloyl beta dextro glucose; catechol methyltransferase; messenger RNA; polyphenol; pyruvic acid; tyrosine; unclassified drug

EMTREE medical terms: amino acid metabolism; antiangiogenic activity; antineoplastic activity; article; breast cancer; cell metabolism; controlled study; gene expression profiling; gene targeting; gluconeogenesis; glycolysis; human; human cell; microarray analysis; nucleotide sequence; poison ivy; real time polymerase chain reaction; Rhus chinensis; tumor gene

MeSH: Angiogenesis Inhibitors; Antineoplastic Agents; Breast Neoplasms; Cell Line, Tumor; Drugs, Chinese Herbal; Female; Genome-Wide Association Study; Glycolysis; Humans; Hydrolyzable Tannins; Microarray Analysis; Molecular Targeted Therapy; Pyruvic Acid; Rhus; Tyrosine
Medline is the source for the MeSH terms of this document.

Species Index: Rhus chinensis

Molecular Sequence Numbers: GENBANK,NM_000051(referenced), NM_000507(referenced), NM_000591(referenced), NM_000629(referenced), NM_000641(referenced), NM_000664(referenced), NM_000754(referenced), NM_000758(referenced), NM_000882(referenced), NM_000920(referenced), NM_000963(referenced), NM_001200(referenced), NM_001202(referenced), NM_001924(referenced), NM_001945(referenced), NM_001951(referenced), NM_002093(referenced), NM_002228(referenced), NM_002648(referenced), NM_002849(referenced), NM_003236(referenced), NM_003238(referenced), NM_004041(referenced), NM_004419(referenced), NM_004591(referenced), NM_004701(referenced), NM_005158(referenced), NM_005228(referenced), NM_005605(referenced), NM_005841(referenced), NM_006850(referenced), NM_013246(referenced), NM_021132(referenced), NM_021135(referenced), NM_021972(referenced), NM_031966(referenced), NM_078467(referenced), NM_134470(referenced), NM_138448(referenced), NM_139034(referenced), NM_139274(referenced), NM_172174(referenced), NM_181353(referenced), NM_206962(referenced)

Chemicals and CAS Registry Numbers: catechol methyltransferase, 9012-25-3; polyphenol, 37331-26-3; pyruvic acid, 127-17-3, 19071-34-2, 57-60-3; tyrosine, 16870-43-2, 55520-40-6, 60-18-4;Angiogenesis Inhibitors; Antineoplastic Agents; Drugs, Chinese Herbal; Hydrolyzable Tannins; Pyruvic Acid, 127-17-3; Tyrosine, 55520-40-6; beta-penta-O-galloyl-glucose, 14937-32-7

ISSN: 10168478 CODEN: MOCEESource Type: Journal Original language: English
DOI: 10.1007/s10059-011-2254-1 PubMed ID: 21614488 Document Type: Article
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