Ethnopharmacological relevance: Atractylodes macrocephala Koidzumi (AMK) is an herbal medicine traditionally used for treatment of abdominal pain, gastrointestinal disease, obesity, and related complications. Aim of the study: We investigated the effects and molecular mechanism of AMK rhizome water extract on 3T3-L1 adipogenesis and an animal model of obesity. Materials and methods: To study the effect of AMK on adipogenesis in vitro, differentiating 3T3-L1 cells were treated every two days with AMK at various concentrations (1-25 μg/ml) for eight days. Oil Red O staining was performed to determine the lipid accumulation in 3T3-L1 cells. To elucidate the inhibitory mechanism of AMK on adipogenesis, phosphorylation levels of Akt and expression of perilipin, were analyzed by Western blotting. AMK was administered orally to high fat diet (HFD)-induced obese rats to confirm its effect in vivo. Results: AMK inhibited 3T3-L1 adipocyte differentiation in a dose-dependent manner without cellular toxicity. Phospho-Akt expression was highly decreased by AMK treatment, whereas there was no significant change in perilipin expression. AMK administration significantly reduced the body weight of rats fed a HFD. Plasma triglyceride levels were significantly lower in the AMK-treated HFD group than those in the HFD control group or normal diet (ND) group, although serum total, HDL- and LDL-cholesterol levels did not differ between the groups. Conclusion: These results demonstrate an inhibitory effect of AMK on adipogenesis through reduction of an adipogenic factor, phospho-Akt. AMK had a beneficial effect, reducing body weight gain in a HFD-induced animal model of obesity. © 2011 Elsevier Ireland Ltd All rights reserved.
3T3-L1 adipocyte; Atractylodes macrocephala Koidzumi; Obesity; Oil Red O staining; Perilipin; Phospho-Akt
EMTREE drug terms: Atractylodes macrocephala extract; cholesterol; high density lipoprotein cholesterol; low density lipoprotein cholesterol; perilipin; protein kinase B; triacylglycerol
EMTREE medical terms: adipocyte; adipogenesis; animal cell; animal experiment; animal model; article; Atractylodes; atractylodes macrocephala; cell differentiation; cell viability; cholesterol blood level; concentration response; controlled study; drug cytotoxicity; enzyme phosphorylation; in vivo study; lipid diet; lipid storage; male; medicinal plant; mouse; nonhuman; obesity; protein expression; rat; rhizome; triacylglycerol blood level; weight reduction; Western blotting
MeSH: 3T3-L1 Cells; Adipocytes; Adipogenesis; Animals; Anti-Obesity Agents; Atractylodes; Blotting, Western; Carrier Proteins; Disease Models, Animal; Dose-Response Relationship, Drug; Male; Mice; Obesity; Phosphoproteins; Phosphorylation; Plant Extracts; Proto-Oncogene Proteins c-akt; Rats; Rats, Sprague-Dawley; Rhizome; Solvents; Staining and Labeling; Time Factors; Triglycerides; Water; Weight Gain
Medline is the source for the MeSH terms of this document.
Species Index: Animalia; Atractylodes macrocephala; Rattus
Chemicals and CAS Registry Numbers: cholesterol, 57-88-5; protein kinase B, 148640-14-6;Anti-Obesity Agents; Carrier Proteins; Phosphoproteins; Plant Extracts; Proto-Oncogene Proteins c-akt, 126.96.36.199; Solvents; Triglycerides; Water, 7732-18-5; perilipin 1