Abstract

It has long been believed that mast cells play a crucial role in the development of many physiological changes during immediate allergic responses. This study was conducted to evaluate the anti-inflammation mechanism of. Schizonepeta tenuifolia (ST) extract and ST purified chemicals on the PMA plus A23187-induced stimulation of HMC-1 human mast cells. ST, rosmarinic acid, pulegone, and 2α,3α,24-thrihydrooxylen-12en-28oic acid treatment of HMC-1 cells led to significant suppression of pro-inflammatory cytokines (IL-6, IL-8, and TNF-α) in a dose dependent manner. In addition, the results of the microarray and real-time RT-PCR analyses revealed that ST regulates several pathways, including the cytokine-cytokine receptor interaction (CCRI), MAPK, and the Toll-like receptor (TLR) signaling pathways. ST may be useful for the treatment of inflammation disease via anti-inflammation activity that occurs through inhibition of the CCRI, MAPK, and TLR signaling pathways. © 2012 Elsevier Inc.

Author keywords

Anti-inflammatory; Cytokine; Microarray; Schizonepeta tenuifolia

Indexed keywords

EMTREE drug terms: antiinflammatory agent; cytokine receptor; interleukin 6; interleukin 8; mitogen activated protein kinase; plant extract; pulegone; rosmarinic acid; Schizonepeta tenuifolia extract; toll like receptor; tumor necrosis factor alpha; unclassified drug

EMTREE medical terms: antiinflammatory activity; article; cell stimulation; controlled study; drug purification; gene expression profiling; human; human cell; inhibition kinetics; mast cell; medicinal plant; microarray analysis; priority journal; protein protein interaction; real time polymerase chain reaction; reverse transcription polymerase chain reaction; signal transduction

MeSH: Anti-Inflammatory Agents, Non-Steroidal; Calcimycin; Calcium Ionophores; Cell Line; Cinnamates; Cytokines; Depsides; Gene Expression Profiling; Humans; Lamiaceae; Mast Cells; Monoterpenes; Oleanolic Acid; Oligonucleotide Array Sequence Analysis; Plant Extracts; Signal Transduction
Medline is the source for the MeSH terms of this document.

Species Index: Schizonepeta tenuifolia

Chemicals and CAS Registry Numbers: interleukin 8, 114308-91-7; mitogen activated protein kinase, 142243-02-5; pulegone, 89-82-7; rosmarinic acid, 20283-92-5; toll like receptor, 409141-78-2;2,3,24-trihydroxyolean-12-en-28-oic acid; Anti-Inflammatory Agents, Non-Steroidal; Calcimycin, 52665-69-7; Calcium Ionophores; Cinnamates; Cytokines; Depsides; Monoterpenes; Oleanolic Acid, 508-02-1; Plant Extracts; pulegone, 15932-80-6; rosmarinic acid, 537-15-5