Abstract

Microglia constitutes the first line of defense against infection and injury in the brain. We previously demonstrated that activated microglia express the Foxp3 gene. However, the role of Foxp3 in cells other than Treg is generally unknown. Therefore, this study was conducted to determine the effects of Foxp3 in LPS-induced activation of microglial cells. The effects of Foxp3 on gene expression profiles in activated microglial cells were evaluated by microarray analysis, which revealed that the Warburg effect, cytokine-cytokine receptor interaction, immune and inflammatory response, Jak-STAT signaling pathway, MAPK signaling pathway, cell adhesion molecules, p53 signaling pathway, and Wnt signaling pathway-related genes were up-regulated in LPS-Treated Foxp3-deficient microglial cells. These findings indicate that Foxp3 might regulate the Warburg effect and inflammatory response in activated microglial cells. © The Korean Society of Toxicogenomics and Toxicoproteomics and Springer 2012.

Author keywords

Foxp3; Gene expression profile; Microglia; Warburg effect

Indexed keywords

EMTREE drug terms: calcium; caspase 6; CD40 antigen; CD82 antigen; CD86 antigen; cell adhesion molecule; chemokine; copper zinc superoxide dismutase; cytokine; fructose bisphosphate aldolase; G protein coupled receptor; guanine nucleotide binding protein; heat shock protein 5; huntingtin; hypoxia inducible factor 1; Janus kinase; Janus kinase 2; lipopolysaccharide; mammalian target of rapamycin; mitogen activated protein kinase; nerve growth factor beta subunit; platelet derived growth factor; prion protein; protein p53; reduced nicotinamide adenine dinucleotide phosphate; STAT protein; stathmin; syndecan 4; transcription factor FOXP3; unclassified drug; Wnt protein

EMTREE medical terms: animal cell; animal cell culture; animal experiment; apoptosis; article; calcium signaling; cell activation; cell cycle; degenerative disease; gene expression profiling; gene expression regulation; glycolysis; Huntington chorea; immune response; inflammation; metabolism; microarray analysis; microglia; molecular interaction; mouse; nonhuman; oxidative phosphorylation; signal transduction; Wnt signaling pathway

Chemicals and CAS Registry Numbers: Janus kinase, 161384-16-3; calcium, 14092-94-5, 7440-70-2; caspase 6, 182372-15-2; copper zinc superoxide dismutase, 149394-67-2; fructose bisphosphate aldolase, 9024-52-6; huntingtin, 191683-04-2; mitogen activated protein kinase, 142243-02-5; reduced nicotinamide adenine dinucleotide phosphate, 53-57-6; stathmin, 126880-56-6; syndecan 4, 479883-59-5