In the present study, we sought to determine whether bee venom (BV) promotes the survival of dopaminergic (DA) neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson's disease (PD). Treatment with BV prevented degeneration of DA neurons in the substantia nigra (SN). This neuro-protective effect of BV was associated with microglial deactivation and reduction of CD4 T cell infiltration. Additionally, BV treatment significantly increased the proportion of CD4+CD25+Foxp3+ Tregs in vivo and in vitro. The increased proportion of Tregs by BV treatment remained suppressive ex vivo. Interestingly, BV treatment did not prevent MPTP neurotoxicity in mice depleted of Tregs by anti-CD25 antibody injection. Therefore, our present studies suggest that modulation of peripheral immune tolerance by Treg may contribute to the neuroprotective effect of BV in the MPTP model of Parkinson's disease. © 2012 Elsevier Inc.
CD4 T cells; Dopaminergic neurons; Microglia; Neuroinflammation; Parkinson's disease; Regulatory T cells
EMTREE drug terms: 1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine; bee venom; CD4 antigen; gamma interferon; interleukin 17; interleukin 2; interleukin 2 receptor alpha; transcription factor FOXP3; tumor necrosis factor alpha
EMTREE medical terms: animal cell; animal experiment; animal model; animal tissue; article; CD4+ T lymphocyte; cell survival; controlled study; dopaminergic nerve cell; ex vivo study; immunological tolerance; immunomodulation; in vitro study; in vivo study; lymphocytic infiltration; male; microglia; mouse; nerve degeneration; neuroprotection; neurotoxicity; nonhuman; Parkinson disease; priority journal; regulatory T lymphocyte; substantia nigra
MeSH: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; Animals; Bee Venoms; Disease Models, Animal; Dopaminergic Neurons; Male; Mice; Mice, Inbred C57BL; Neuroprotective Agents; Parkinson Disease; T-Lymphocytes, Regulatory
Medline is the source for the MeSH terms of this document.
Chemicals and CAS Registry Numbers: 1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine, 28289-54-5; gamma interferon, 82115-62-6; interleukin 2, 85898-30-2;1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, 28289-54-5; Bee Venoms; Neuroprotective Agents