Abstract

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system that leads to substantial disability through deficits of sensation and of motor, autonomic, and neuro-cognitive function. Many clinical and pathological features of experimental autoimmune encephalomyelitis (EAE) show close similarity to MS. Bee venom (BV) has been used in the practice of oriental medicine and evidence from the literature indicates that BV plays an anti-inflammatory or anti-nociceptive role against inflammatory reactions associated with arthritis and other inflammatory diseases. The purpose of the present study was to determine whether BV could suppress immune cell differentiation and infiltration into spinal cord on EAE mice commonly used as a model for MS. BV treatment increased the population of CD4⁺CD25 ⁺Foxp3⁺ T cells and inhibited CD4⁺ T-cell proliferation in vitro. In vivo, BV treatment increased the population of CD4⁺CD25⁺Foxp3⁺ T cells. Furthermore, BV administration reduced the severity of EAE while concurrently decreasing INF-γ producing CD4⁺ T cells, IL-17A producing CD4⁺ T cells and inflammatory cytokine production including INF-γ, IL-17A, TNF and IL-6. BV-treated animals exhibited less infiltration and preserved morphology compared to saline-treated animals. Interestingly, the therapeutic effects of BV on EAE disappeared when CD4⁺CD25⁺Foxp3 ⁺ T cells were depleted by using anti-CD25 antibody. Our research suggests that BV could be a potential therapeutic agent for anti-inflammatory effects in an animal model of EAE. Copyright © by BIOLIFE, s.a.s.

Author keywords

Bee venom; EAE/MS; Immunology; Inflammation

Indexed keywords

EMTREE drug terms: bee venom; gamma interferon; interleukin 17; interleukin 6; transcription factor FOXP3; tumor necrosis factor

EMTREE medical terms: allergic encephalomyelitis; animal experiment; animal model; animal tissue; article; CD25+ T lymphocyte; CD4+ T lymphocyte; controlled study; cytokine production; drug effect; experimental infection; female; lymphocyte differentiation; lymphocyte subpopulation; lymphocytic infiltration; mouse; nonhuman

Chemicals and CAS Registry Numbers: gamma interferon, 82115-62-6

Manufacturers:Drug manufacturer: Sigma Aldrich, United States.