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기타논문
Date: March 2013

Journal: International Immunology Volume 25, Issue 3, March 2013, Pages 197-205 , Doi: 10.1093/intimm/dxs102

2013 | Vitex rotundifolia L. Prevented airway eosinophilic inflammation and airway remodeling in an ovalbumin-induced asthma mouse model…

Bae, H.a, Kim, Y.a, Lee, E.b, Park, S.a, Jung, K.-H.a, Gu, M.-J.c, Hong, S.-P.c, Kim, J.b

Abstract

Vitex rotundifolia L. (VR) as long been used in China and Korea in traditional medicine. This study was conducted to evaluate the ability of Vitex rotundifolia L. to prevent airway inflammation and remodeling in an ovalbumin (OVA)-induced murine asthma model. The total cell number and number of inflammatory cells in the bronchoalveolar lavage (BAL) fluid were counted. The levels of cytokines in the BAL fluid and serum IgE levels were measured using an ELISA. For histological analysis, hematoxylin and eosin staining, periodic acid-Schiff staining and immunohistochemistry were evaluated. The release of total cells into the BAL fluid was significantly inhibited in OVA-induced asthmatic mice treated with VR extract. In addition, eosinophilia and lymphocytosis were reduced significantly in mice that received VR extract. Furthermore, levels of the Th2 cytokines IL-4 and IL-5 and pro-inflammatory cytokine TNF-α in the BAL fluid and total IgE in serum were markedly suppressed by VR extract. OVA-specific IgE in the serum and IL-13 in the BAL fluid were decreased, but not significantly. The allergic effects of VR extract were accompanied by a reduction in airway hyperresponsiveness. Additionally, morphologic findings demonstrated that VR extract substantially inhibited OVA-induced eosinophilia, goblet cell hyperplasia and smooth muscle mass production. This finding suggests that VR extract may have pharmacological effects that would be useful for the treatment of asthma via the inhibition of the Th2 response and airway remodeling. © The Japanese Society for Immunology. 2012. All rights reserved.


Author keywords

Airway hyperresponsiveness; Eosinophilia; Goblet cell hyperplasia; Smooth muscle actin; Th2 response


Indexed keywords

EMTREE drug terms: dexamethasone; eosin; hematoxylin; immunoglobulin E; interleukin 4; interleukin 5; ovalbumin; tumor necrosis factor alpha; Vitex rotundifolia extract

EMTREE medical terms: airway remodeling; animal cell; animal experiment; animal model; animal tissue; article; asthma; cell count; cell hyperplasia; cell structure; controlled study; drug mechanism; enzyme linked immunosorbent assay; eosinophilia; goblet cell; histopathology; immunohistochemistry; lung lavage; lymphocytosis; mouse; nonhuman; periodic acid Schiff stain; priority journal; respiratory tract inflammation; smooth muscle; Vitex rotundifolia

MeSH: Airway Remodeling; Animals; Asthma; Cell Movement; Cells, Cultured; Cytokines; Disease Models, Animal; Eosinophils; Humans; Immunoglobulin E; Inflammation; Male; Medicine, Chinese Traditional; Mice; Mice, Inbred BALB C; Ovalbumin; Plant Extracts; Respiratory System; Th2 Cells; Vitex
Medline is the source for the MeSH terms of this document.


Chemicals and CAS Registry Numbers: dexamethasone, 50-02-2; eosin, 17372-87-1, 51395-88-1, 548-26-5; hematoxylin, 517-28-2; immunoglobulin E, 37341-29-0; ovalbumin, 77466-29-6;Cytokines; Immunoglobulin E, 37341-29-0; Ovalbumin, 9006-59-1; Plant Extracts


ISSN: 09538178 CODEN: INIMESource Type: Journal Original language: English
DOI: 10.1093/intimm/dxs102 PubMed ID: 23171813 Document Type: Article
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