Abstract

The present study investigated the antiobesity effect of Achyranthes bidentata Blume root water extract in a 3T3-L1 adipocyte differentiation model and rats fed with a high-fat diet. To investigate the effect of Achyranthes bidentata Blume on adipogenesis in vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated every two days with Achyranthes bidentata Blume at various concentrations (1 to 25 g/mL) for eight days. We found that Achyranthes bidentata Blume root inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, and Western blot analysis revealed that phospho-Akt expression was markedly decreased, whereas there was no significant change in perilipin expression. Furthermore, administration of Achyranthes bidentata Blume root (0.5 g/kg body weight for six weeks) to rats fed with a high-fat diet significantly reduced body weight gain without affecting food intake, and the level of triglyceride was significantly decreased when compared to those in rats fed with only a high-fat diet. These results suggest that Achyranthes bidentata Blume root water extract could have a beneficial effect on inhibition of adipogenesis and controlling body weight in rats fed with a high-fat diet. © 2014 Sang Deog Oh et al.

Indexed keywords

EMTREE drug terms: Achyranthes bidentata extract; antiobesity agent; perilipin; protein kinase B; triacylglycerol

EMTREE medical terms: Achyranthes bidentata; adipocyte; adipogenesis; animal cell; animal experiment; animal model; article; cell differentiation; cell viability; enzyme phosphorylation; food intake; lipid diet; lipid storage; lipolysis; male; nonhuman; proadipocyte; protein expression; rat; weight reduction; Western blotting

Chemicals and CAS Registry Numbers: protein kinase B, 148640-14-6