Date: 5 December 2014

Journal: PLoS ONE Volume 9, Issue 12, 5 December 2014, Article number e114726 , Doi: 10.1371/journal.pone.0114726

2014 | Bee Venom Phospholipase A2 Protects against Acetaminophen-Induced Acute Liver Injury by Modulating Regulatory T Cells and IL-10 in Mice…

Hyunseong Kim, Dong June Keum, Jung won Kwak, Hwan-Suck Chung,Hyunsu Bae



The aim of this study was to investigate the protective effects of phospholipase A2 (PLA2) from bee venom against acetaminophen-induced hepatotoxicity through CD4+CD25+Foxp3+ T cells (Treg) in mice. Acetaminophen (APAP) is a widely used antipyretic and analgesic, but an acute or cumulative overdose of acetaminophen can cause severe hepatic failure. Tregs have been reported to possess protective effects in various liver diseases and kidney toxicity. We previously found that bee venom strongly increased the Treg population in splenocytes and subsequently suppressed immune disorders. More recently, we found that the effective component of bee venom is PLA2. Thus, we hypothesized that PLA2 could protect against liver injury induced by acetaminophen. To evaluate the hepatoprotective effects of PLA2, C57BL/6 mice or interleukin-10-deficient (IL-102/2) mice were injected with PLA2 once a day for five days and sacrificed 24 h (h) after acetaminophen injection. The blood sera were collected 0, 6, and 24 h after acetaminophen injection for the analysis of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). PLA2-injected mice showed reduced levels of serum AST, ALT, proinflammatory cytokines, and nitric oxide (NO) compared with the PBS-injected control mice. However, IL-10 was significantly increased in the PLA2-injected mice. These hepatic protective effects were abolished in Tregdepleted mice by antibody treatment and in IL-102/2 mice. Based on these findings, it can be concluded that the protective effects of PLA2 against acetaminopheninduced hepatotoxicity can be mediated by modulating the Treg and IL-10 production. Copyright:

Indexed keywords

Species Index: Apoidea; Mus

ISSN: 19326203 CODEN: POLNCSource Type: Journal Original language: English
DOI: 10.1371/journal.pone.0114726 Document Type: Article
Publisher: Public Library of Science
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