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Adenoviral gene transfer of acetylcholinesterase T subunit in the hypothalamus potentiates electroacupuncture analgesia in rats

  • AD 최고관리자
  • 조회 3152
  • 2013.08.19

Keywords:

  • Acetylcholinesterase;
  • adenovirus;
  • electroacupuncture analgesia;
  • hypothalamus;
  • microarray;
  • non-responder;
  • rats;
  • real-time RT-PCR;
  • responder

Our previous studies, using cDNA microarray and real-time reverse transcription-polymerase chain reaction, showed that acetylcholinesterase T subunit (AChET) gene was more abundantly expressed in the hypothalamus of the responder rats that were sensitive to electroacupuncture (EA) in the tail flick latency (TFL) test than in that of the non-responder rats that were insensitive to EA. In this study, we hypothesized that the expression of the AChET gene in the hypothalamus modulates EA analgesia in rats. To explore the hypothesis, we constructed an AChET-encoding adenovirus and a control virus expressing only green fluorescence protein, either of which was then injected into the hypothalamus of Sprague-Dawley rats. The hypothalamic activity of acetylcholinesterase was significantly higher in rats that were injected with the AChET virus than in rats that were injected with the control virus. The basal pain threshold measured by a TFL test was not changed by microinjection of AChET or control virus into the hypothalamus when EA treatment was not conducted. However, the analgesic effect of EA was significantly enhanced from 7 days after microinjection of the AChET virus into the hypothalamus but not after injection of the control virus. Furthermore, expression of the AChET in the hypothalamus did not affect body core temperature, body weight, motor function or learning and memory ability. Taken together, these results suggest that adenoviral expression of the AChET gene in the hypothalamus potentiates EA analgesia in rats without apparent side-effects.


TITLE
 Adenoviral gene transfer of acetylcholinesterase T subunit in the hypothalamus potentiates electroacupuncture analgesia in rats 
JOURNAL
 Genes, Brain and Behavior
 Volume 8, Issue 2, March 2009, Pages 174-180
 DOI
 10.1111/j.1601-183X.2008.00459.x (click 하시면 DOI 검색 결과가 새창으로 나타납니다.)
 
트위터 페이스북 미투데이 다음요즘 싸이공감 카카오톡 카카오스토리 네이트온 쪽지 구글 북마크 네이버 북마크

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